Summer research symposium to feature INBT-hosted interns

The School of Medicine will host the second annual Hopkins Career Academic and Research Experiences for Students (C.A.R.E.S.) Summer Symposium on Thursday, July 30, from 10 a.m. to 3:30 p.m.in the Anne and Mike Armstrong Medical Education Building.

SOM150502 CARES Summer 2015 Program Poster 24x36-3 (1)_Page_2Johns Hopkins Institute for NanoBioTechnology has 15 Research Experience for Undergraduates participating in the symposium.  In addition to more than one dozen poster presenters, REU Ashley Williams will give an individual talk on her research project at 1:20 p.m. in the East Auditorium. High school students from the INBT supported SARE program (Summer Academic Research Experience) will also have posters, and two-time SARE scholar Assefa Akinwole will give a talk on his work at 12:50 p.m. in the West Auditorium. The symposium is free and open to the entire Hopkins Community.

In total, more than 150 high school students from Baltimore City and undergraduates from around the country will present posters and oral presentations. Peter Agre, M.D. (Med ’74), director of the Johns Hopkins Malaria Research Institute, will deliver the keynote address.

“This is an excellent opportunity for our Baltimore City scholars to showcase their talents, intellect, and passion for science and medicine and reaffirm that they can compete at the highest level with undergraduates from across the country,” said Danny Teraguchi, Ph.D., assistant dean for student affairs and director of the office for student diversity.

C.A.R.E.S. is grateful to the Office of the Vice Dean for Education, Pulmonary and Critical Care Medicine Summer Internship Program, Johns Hopkins Internship Program in Brain Sciences, and its corporate sponsor, PNC, for supporting the symposium, and for their commitment to advancing education opportunities and academic programming for Baltimore City youth.

All press inquiries about this program or about INBT in general should be directed to Mary Spiro, INBT’s science writer and media relations director at mspiroATjhu.edu.

 

Assefa Akinwole

Assefa Akinwole

Ashley Williams

Ashley Williams

REU Profile: Hydrogels and stem cells

FranklynHall

Franklyn Hall

Franklyn Hall is a rising junior at Mississippi State University where he is studying Chemical Engineering with a Biomolecular Concentration. He is spending the summer in the chemical and biomolecular engineering laboratory of Sharon Gerecht as part of the Johns Hopkins Institute for NanoBioTechnology Research Experience for Undergraduates program (INBT REU).

Franklyn wanted to write about his experience thus far at Johns Hopkins in the INBT REU program in a blog post as follows:

This summer at the INBT REU has been an amazing experience that has allowed me to investigate interesting research topics such as hydrogels and stem cell growth. This experience has also given me the opportunity to learn more about the JHU community and the life of a graduate student.

My research is mainly focused on the characterization of the optimal conditions for vascular regeneration and growth within hydrogels. Hydrogels are unique 3-D environments that mimic in-vivo cell growth and allow researchers to study and adjust growth conditions, patterns, and cell interactions. These 3-D growth environments not only improve our understanding of stem cells, but they have applications in wound healing and tissue regeneration. I am specifically investigating hypoxia in hydrogels or the state of having low oxygen availability within the hydrogel. One of my research goals is to find the optimal hypoxic conditions and the effect of oxygen gradients within the hydrogel on cell growth and development. I have enjoyed learning how to make the hydrogel polymers, culture and stain cells, and look forward to producing results soon.

Outside of the laboratory I have had the opportunity to play on the departmental softball team with my graduate student mentor. It is common for graduate students to play different sports in the evening to socialize and have fun outside of the laboratory. During our semiweekly games, I have been able to talk to Masters, MD, and MD/PhD. students to learn about their graduate study experiences and future goals.  We have also had the opportunity to go out to eat and go to different events around Baltimore.

Graduate studies and research may be challenging. However, with people like the ones I have met, the support is there for you to persevere and make your mark on the scientific community.

All press inquiries about this program or about INBT in general should be directed to Mary Spiro, INBT’s science writer and media relations director at mspiroATjhu.edu.

 

High school students join ranks of summer researchers at INBT

Twelve high school students have joined the ranks of researchers working in labs affiliated with Johns Hopkins Institute for NanoBioTechnology this summer.

Nine of the students come to Hopkins via the Summer Academic Research Experience program (SARE), which pairs selected academically inclined students who attend Baltimore area schools, such as the SEED School of Maryland, with laboratory mentors at the Johns Hopkins School of Medicine. INBT has administered this program since 2009. Its funding comes from a variety of sources including, INBT, the Family League of Baltimore and private grants and donations.

SARE-2015-kids-web

SARE 2015 Cohort: Back row: Natalie Suarez-Perez, Adam Elsaidy, Princess Massaquoi, Ayende Watson, Stacey Alston. Front row: Assefa Akinwole, Siri Keyaka, Tashanna Sands, Grace Ayole,

Three of the students have joined INBT labs through a supplement to the National Science Foundation’s REU (Research Experience for Undergraduates) program and these students have been placed in INBT affiliated laboratories on the Homewood campus of the University.  This program is new to the Institute and students selected had applied directly to NSF via school recommendations. Students in this program come from outside the Baltimore area and live on campus in student housing.

INBT is dedicated to providing educational outreach opportunities to a variety of populations, especially students living in and around the Johns Hopkins University campuses. To find out about all of our educational program, visit the website http://inbt.jhu.edu/education/. Questions about our academic programs may be directed to INBT’s Academic Program Administrator Camille Bryant at cbryantATJHU.edu

All press inquiries about this program or about INBT in general should be directed to Mary Spiro, INBT’s science writer and media relations director at mspiroATjhu.edu.

NSF High School Cohort 2015: Nico Deshler, Nahom Yimam and Prathak Naidu

NSF High School Cohort 2015: Nico Deshler, Nahom Yimam and Prathak Naidu

REU student profile: Christopher Glover

Christopher Glover is a rising senior in bioengineering at the University of Missouri. He worked this summer as an REU intern in the laboratory of professor Jeff Tza-Huei Wang, who has joint appointments in mechanical engineering, biomedical engineering and oncology. The Research Experience for Undergraduates, hosted by Johns Hopkins Institute for NanoBioTechnology, attracts nearly 800 undergraduate applicants for just 10 research positions.

Christopher Glover

Christopher Glover

Christopher’s project involved a proof-of-concept experiment to test a device used to digitally sort and amplify DNA samples.

The device consists of a silicone chip imprinted with 3,000 tiny wells to contain DNA. A thermoplastic lid covers the top of the chip to keep the DNA in place in the wells. After a segment of DNA is added to the chip, the number of copies of that DNA segment is amplified using a device called a thermal cycler. “The goal is to either get zero or one copy of the DNA segment in each well, which makes the device “digital,” he said.

“We aren’t concerned about the type of DNA we are amplifying but just to see if it will work,” Christopher said. “This could be used for medical screening where a specific allele could be detected within a gene to see if someone is more susceptible to getting a disease,” he said.

Christopher said that working in the Wang lab has helped him learn much more about nanotechnology than he had previously known. His future plans include earning a PhD in biomedical engineering.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

REU student Profile: Florencia Velez-Cortes

Florencia Velez-Cortes is a rising senior in physics and chemistry at The Ohio State University. As part of INBT’s REU program, she spent her summer as a research intern the chemical and biomolecular engineering laboratory of professor David Gracias. REU stands for Research Experience for Undergraduates and is a National Science Foundation program hosted by the Johns Hopkins Institute for NanoBioTechnology.

Florencia Velez-Cortes

Florencia Velez-Cortes

Florencia worked constructing bi-layers out of DNA and acrylamide gel. The combination of the two materials could be used the make biocompatible devices, such as microgrippers that could be used in tether-less surgery, she explained.

“The best part about this project is that we are working on something complete new and biocompatible that could even be responsive to chemical signals,” she explained.

Also because the material is composed of DNA, “it could be responsive to certain DNA sequences.”

Florencia said the most challenging thing for her this summer was working with people who are engineers, when she is not. “It’s been a steep learning curve for me but everyone has been really helpful and receptive to working with someone who is outside of their field.”

She also noted that the pace of research in the Gracias lab is a lot faster than what she is used to in her previous laboratory experiences. She said having a mentor she could talk to was integral to her success.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

REU student profile: Alexa Wnorowski

Alexa Wnorowski

Alexa Wnorowski

Alexa Wnorowski is a rising senior in Biological Engineering studying at Cornell University. Her summer REU at Johns Hopkins University involved studying the blood brain barrier in the materials science and engineering laboratory of Peter Searson. Searson directs the Institute for NanoBioTechnology that hosts the Research Experience for Undergraduates (REU).

“The goal is to figure out how to move drugs across this tight junction of cells that protects the brain,” Alexa explained. Alexa used a four-channel microfluidic device fabricated in the Searson lab.

“Each channel has a slightly different shear stress because it has a slightly different height,” she explained. “We seed a culture of cells at the bottom of the channel and let them grow so that they are confluent. Then we run media through the device and we see how the different shear stresses affects proliferation of the cells.”

Although she conducted research before, Alexa said she had never worked with microfluidic devices before. She said to gain a broader perspective on research, it’s important to work in several different laboratories. “Every lab is run in different ways and have very different atmospheres, “ she said. “This experience has shown me how research in conducted in different labs.”

Alexa’s goal is to earn a PhD in biomedical engineering.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

 

REU student profile: Rebecca Majewski

DNA, the genetic sequence that tells cells what proteins to manufacture, typically resides inside the nucleus of a cell, but not always. Rebecca Majewski is studying the uptake of DNA into cell nuclei using a different polymer chains. Rebecca is a rising senior in BioMolecular Engineering from the Milwaukee School of Engineering and is working as a summer intern in the Johns Hopkins Institute for Nanobiotechnology’s REU program.

“We are interested in how much of the DNA with the polyplex can get into the nucleus,” she said, but explains that DNA associated outside of the nucleus can cause false higher measurements.

Rebecca Majewski. Photo by Mary Spiro

Rebecca Majewski. Photo by Mary Spiro

Rebecca is washing the cells with the nuclei to get rid of DNA outside the nucleus and then comparing the measurement of uptake of the DNA by the cell versus the measurement of the uptake of DNA by the nucleus.

“We are interested in what DNA gets inserted into the nucleus because that is what is ultimately expressed. It is important to find out how much makes it to the final destination and then is expressed. The goal of this work is to test different polymer chains to see which one actually does the better job of getting the DNA into the nucleus,” she said.

Rebecca works alongside PhD students and postdoctoral fellows in the biomedical engineering lab of Jordan Green lab at the Johns Hopkins School of Medicine. She says she highly values the opportunity for a research experience through INBT’s REU because her undergraduate institution does not train graduate students.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

REU student profile: Ian Reucroft

Sitting at what looks like a pottery wheeled turned on its side, Ian Reucroft is using a method called electrospinning to create a nano-scale polymer fiber embedded with a drug that encourages nerve growth. The strand is barely visible to the eye, but the resulting fibers resemble spider web.

Ian Reucroft, a rising junior in Biomedical Engineering at Rutgers University, is working in the medical school campus laboratory of Hai-Quan Mao, professor of materials sciences and engineering at Johns Hopkins University. He is part of Johns Hopkins Institute for NanoBioTechnology’s summer REU, or research experience for undergraduates program.

Ian Reucroft in the Mao lab. Photo by Mary Spiro.

Ian Reucroft in the Mao lab. Photo by Mary Spiro.

“We are developing a material to help regrow nerves, either in central or peripheral nervous systems,” said Ian. One method of doing that he explained is to make nanofibers and incorporating a drug into those fibers, drugs that promote neuronic growth or cell survival or various other beneficial qualities. The Mao lab is looking into a relatively new and not well-studied drug called Sunitinib that promotes neuronal survival.

“We make a solution of the component to make the fiber, which is this case is polylactic acid (PLA), and the drug, which I have to dissolve into the solution,” Ian said. Although the drug seems to remain stable in solution, one of the challenges Ian has faced has been improving the distribution of the drug along the fiber.

This is Ian’s first experience with electrospinning but not his first time conducting research. He plans to pursue a PhD in biomedical engineering and remain in academia.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

REU student profile: Sierra Atwater

Sierra Atwater just completed her freshman year as a biology major at the University of North Carolina Chapel Hill. She works in the chemical and biomolecular engineering laboratory of professor Denis Wirtz at Johns Hopkins University and is part of the Institute for NanoBioTechnology REU program. REU stands for Research Experience for Undergraduates and is a National Science Foundation funded program administered by INBT.

Sierra explained that the Wirtz group is “looking at cell motility and proliferation to see how different cancer cells move and what affects that movement.”

Sierra Atwater

Sierra Atwater

“I’ve been looking at cell division and cell density over a 16-hour period,” she added. “I have learned how to do a lot in this lab such as how to make 3D collagen gel matrices and how to make 2D gels, how to use T2000 microscopes and the confocal microscopes and how to do ELISAs,” which are enzyme-linked immunosorbent assays or tests use antibodies to detect substances.

Hasini Jayatilaka, PhD student in ChemBE, mentors Sierra. In addition, Sierra says she feels she can call upon the expertise of other students working in the Wirtz lab. This is her first research experience, and she says, the hardest thing so for has been just remembering how to do so many different techniques.

For more information about INBT’s summer REU program, visit this link.

For all press inquiries regarding INBT, its faculty and programs, contact Mary Spiro, mspiro@jhu.edu or 410-516-4802.

 

 

 

One REU’s experience at the Council on Undergraduate Research Conference

Over this past summer, I participated in the Research Experience for Undergraduates Program at Johns Hopkins University Institute for NanoBioTechnology (INBT). I was placed in Douglas Robinson’s laboratory under the mentorship of Vasudha Srivastava in the School of Medicine. I worked on the preliminary studies necessary to determine the molecular mechanisms of regulation within the cell’s mechanosensory system.

It was one of the best experiences of my life; I thoroughly enjoyed every day of it. Dr. Robinson and his lab members were extremely welcoming, supportive, and resourceful throughout the entire ten weeks and even after the program ended. I was able to gain substantial research experience and develop relationships with the people in the lab. At the end of the program, I was fortunate enough to be chosen to present the work I had done at the 2013 Council on Undergraduate Research Conference of Research Experiences for Undergraduates Student Scholarship (CUR CREUSS) in Arlington, VA.

Shantel Angstadt, a 2013 REU, during the INBT poster session. Photo by Mary Spiro.

The CUR CREUSS was another great experience. I was able to meet students who participated in REU programs at institutions from all across the country as well as the faculty members involved in the REU programs. Each student participated in three, 30-minute poster sessions, and we were asked questions about our work and overall experience during the summer.

We were also able to attend a faculty poster session during which each faculty member presented the work he or she is currently involved in. I loved having the opportunity to talk about my experience and to learn of others’ experiences doing research. There was a very diverse group of studies among the students and faculty in a variety of fields, such as: chemistry, engineering, life sciences, mathematical sciences, geosciences, psychology, social sciences, and materials research. The conference also provided “Breakout Sessions” in which National Science Foundation (NSF) members presented on the importance of research, good conduct, graduate school, careers, research funding, and a variety of other topics. These sessions were very informative and useful. I attended the “Applying for Graduate School Support from NSF” and “Incorporating Your Research Experiences into Applications for Post-Baccalaureate Fellowships and Nationally Competitive Awards” sessions. Overall, this experience allowed me to further develop my presentation skills, gain exposure to other fields of study, network, and learn of all the resources provided by the NSF.

One last piece of information from the CUR CREUSS that I feel the need to share with other undergraduate students is of the NSF Graduate Research Fellowship Program. It provides three years of financial support to graduate students and, if awarded, makes the student more competitive when applying to graduate programs. It is a great opportunity that I was not aware of until attending the conference.

My experiences from the INBT REU program and CREUSS have lead me to believe that it should be mandatory for students interested in research careers to participate in extracurricular research programs. I gained information and experience that are essential in choosing a career and developing as a scientist and as a person. I believe the most important aspects of each were the exposure to different fields of study and the information on the different resources and opportunities available to undergraduates. I will always be grateful for these opportunities provided by the INBT faculty and staff and Dr. Robinson’s lab.

Shantel Angstadt is in her senior year at Elizabethtown College studying biochemistry and was an REU intern at Johns Hopkins Institute for NanoBioTechnology for the summer of 2013.