Johns Hopkins Integrated Imaging Center focuses on data

Shyenne Yang positions Drosophila embryos for fluorescence imaging. Photo by Marty Katz/baltimorephotographer.com

Heavy, black curtains and dimmed lights shroud the core of the Johns Hopkins Integrated Imaging Center (IIC). Yet researchers who peer through the advanced microscopes cloaked by these dark draperies view experimental samples more clearly than ever thanks to a combination of the high-tech equipment and the creative expertise offered by the center’s seven-member staff.

When describing Johns Hopkins University’s showpiece microscopy facility, it’s easy to rattle off a laundry list of available equipment and laboratory space able to prepare samples with nearly any contrasting agent found in the literature. The Homewood-based center contains devices that can image a sample in virtually any manner in 2-D, 3-D and even 4-D. IIC’s 3,500 square-foot facility comprising space in Dunning, Jenkins, and Olin Halls, boasts more than $7.5 million worth of state- of-the-art imaging equipment, including a Zeiss laser scanning microscope (LSM) 510 VIS confocal with a Confocor 3 fluorescence correlation spectroscopy (FCS) module—one of only a very few such uniquely configured laser scanning microscopes in the United States.

Director J. Michael McCaffery, a research professor in the Department of Biology at the Krieger School of Arts and Sciences, said the Hopkins community is thrilled to have access to such a versatile microscope with fluorescence correlation spectroscopy that is capable of cross-correlation analysis, with confocal imaging and a fully enclosed environmental system for live imaging. Researchers affiliated with Johns Hopkins Institute for NanoBioTechnology (INBT), the Johns Hopkins Physical Sciences Oncology Center and Center of Cancer Nanotechnology Excellence are also glad to have access to IIC’s menu of facilities.

“Fluorescence correlation spectroscopy allows for high-resolution spatial and temporal analysis of single biomolecules with respect to diffusion, binding, as well as enzymatic reactions in vitro and in vivo,” McCaffery said. In other words, you can see and measure a lot of really tiny stuff with it, something INBT affiliated researchers working at micron/nanometer resolutions are finding incredibly useful.

The center features multiple suites devoted to specific microscopy/imaging functions, as well as facilities for all manner of sample preparation. All these advanced tools help scientists and engineers characterize nanomaterials; and image cells, sub-cellular organelles, and biomolecules/ proteins at very small dimensions. But none of this fancy equipment would be of much use to researchers without the expertise of McCaffery and the IIC staff. McCaffery brings years of experience and a background in cell biology and microbiology. The center’s associate director, William Wilson, an associate research professor in the Department of Materials Science and Engineering at the Whiting School of Engineering, describes himself as a “chemist, turned physicist, who became an electrical engineer, who is now a materials scientist.”

Staff scientist Kenneth J.T. Livi, director of the IIC’s High-Resolution Analytical Electron Microbeam Facility located in Olin Hall, offers his unique perspective on earth and planetary sciences. Researchers can also consult with microscopy specialist/ trained biologist and FACS supervisor Erin Pryce, the FACS manager Yorke Zhang, computer/IT specialist Marcus Sanchez, and research assistants Leah Kim and Adrian Cotarelo, who both are currently earning their bachelor degrees in biology at Johns Hopkins.

From left, IIC director Michael McCaffery, FACS supervisor Erin Pryce, and associate director William Wilson with the BD FACSVantage SE. Photo by Mary Spiro

“Sometimes young researchers haven’t contemplated all the possibilities of how to use and apply an instrument; and don’t realize there are many different ways to utilize familiar tools in order to obtain new, in some cases better, information,” McCaffery said. “Our desire is always to approach a problem from many disparate perspectives to generate convergent data that corroborates each particular assay. Hopefully, results from each individual assay, allows the scientist to arrive at a convergent perspective that yields confidence in the results and conclusions.”

One of the easiest ways to obtain different microscopy data and improve corroboration among assays is simply to change the contrast mechanism.

“The most common contrast mechanisms used to image something are optical contrast (transparent versus opaque), polarization, and fluorescence,” said Wilson. “But there are many different ways you can manipulate how light interacts with the specimen and what you detect out of an objective.”

For example, ultrafast laser sources have made nonlinear optical forms of contrast an exciting new tool. Techniques like two-photon excited fluorescence and second harmonic generation (both available in the IIC) produce excellent spectral and structural information about samples because a smaller effective photon volume is excited. Wilson explained it like this: “Imagine turning your stereo all the way up and hearing the sound distorted. That distortion is created by the higher order acoustic harmonics from your stereo. The same happens with intense laser light resulting in new “colors” being generated from the object irradiated. The cool thing is that the different non-linear processes are often sensitive to different physical proper- ties or structural features, offering complementary information about your sample.”

In some cases, getting more detailed information simply requires looking at the right color range. The two-photon fluorescence and second harmonic signals appear at different wavelengths. If you excite a sample with enough energy to generate third order harmonics, that signal is detected at an even bluer wavelength, Wilson said. “With third harmonic generation, you only get signals from the interface of structures with no interference from anything else. This means you can simultaneously image fluorescence, polar order, and interface dynamics just by popping in a few filters and beam splitters,” he said.

“Over the past ten or so years, physicists and engineers focused on advanced microscopy, have produced better and more advanced laser and optical technologies, generating techniques that many researchers in the biological and biomedical sciences might not know exist,” Wilson said. “There also are a lot of applied physicists who are developing and using these new technologies who don’t know what an interesting sample is. We hope to help bridge this gap, becoming a place where these collaborative synergies can flourish.”

Sample preparation is another area where the center can help researchers. “Cell fractionation, for example, which is the breaking down of whole cells and separating them into their individual components, when combined with biochemical techniques and microscopy, can often allow researchers to pose more precise questions and to better analyze a biological problem,” McCaffery said.

“It is common for someone to come in and want to use a particular instrument or technique they read about in a paper,” McCaffery said. When that happens, McCaffery and Wilson are likely to give researchers “homework.”

“It’s important to remember that the goal is not to make a pretty picture,” Wilson said. “The goal is to answer a question, so sometimes we have to ask them, ‘What is your research question?’” An enviable set of microscopy tools combined with a team that brings years of training and experience from a variety of disciplines sets Johns Hopkins Integrated Imaging Center apart from the microscope on the individual researcher’s lab bench, as well as from facilities nationwide. Wherever possible, McCaffery said, IIC staff tries to be engaged in all of the research that is carried out in the center. “Simply, our involvement leads to better results and better science,” McCaffery added.

Researchers confirm this successful combination.

“The facilities at the IIC have allowed us to obtain critical information about the internal structure of our peptide nanomaterials that would have remained unknown without careful electron and fluorescence microscopy,” said J.D. Tovar, assistant professor of Chemistry. “Equally important, the scientific IIC staff members were vital participants making sure collaborative experiments were done meaningfully and students were trained competently. Our collaboration with Dr. Wilson has given some nice insights and at the same time has posed many more questions for future research.”

Praise like that for the IIC is always nice to hear, staff members say, but they emphasize that the services and tools they provide are just part of the job. “Part of being a scientist is learning not only how to gather information from a wide variety of tools but also understanding how to pose clear questions that lead to the right tools, in a nutshell, how to not waste time. If we can help you do that, then we have achieved our goal,” Wilson said.
This story originally appeared in Johns Hopkins Nano-Bio Magazine.

To read more about IIC’s facilities and services, go here.

Story by Mary Spiro

Photos by Mary Spiro and Marty Katz

 

Sponsors needed for JHU nano-bio symposium

Andrew Wong and Noah Tremblay peruse the first issue of NanoBio Magazine (Photo by Charli Dvoracek/INBT)

Cancer Nanotechnology is the theme of the fifth annual symposium of Johns Hopkins Institute for NanoBioTechnology (INBT), May 12-13, 2011 at the university’s Homewood campus. Sponsors are needed to help offset the cost of publishing Nano-Bio magazine, which serves as the event’s program and to provide prizes for top poster presenters. The poster session will feature at least 80 research posters from INBT affiliated research laboratories.

If you or your organization would like to learn how to sponsor the INBT’s annual symposium, please contact our director of corporate partnerships, Tom Fekete, at tmfeke@jhu.edu or call him at 410-516-8891. Sponsors enjoy reduced rates on symposium-related events and advertising in our annual Nano-Bio magazine/symposium program, among other benefits.

Additionally, INBT also needs sponsors to donate prizes for the poster session. Books, gift cards, science-themed t-shirts and the like all make wonderful prizes for our student researchers. If your organization would like to donate a prize, please contact INBT’s science writer Mary Spiro at mspiro@jhu.edu or 410-516-4802.

For more details on the symposium, including a list of speakers, click here or go to http://inbt.jhu.edu/outreach/symposium/twentyeleven/

To learn more about sponsorship, click here or go to http://inbt.jhu.edu/outreach/symposium/twentyeleven/sponsorship-information/

Cancer Nanotechnology theme of INBT’s symposium, May 12-13

The Denis Wirtz lab research centers on investigations of cell micromechanics, cell architecture, nuclear shape and gene expression. Shown are healthy mouse cells with flurorescent staining of the nucleus (blue) and microtubules (green) emanating from the microtubule organizing center (red). (Photo: Wirtz Lab/JHU)

Nanoscale tools developed by engineers have yet to be fully explored and exploited for the diagnosis and treatment of diseases such as cancer. Nanotechnology for Cancer Medicine forms the focus of the fifth annual symposium for Johns Hopkins Institute for NanoBioTechnology (INBT), May 12 and 13, 2011 at the university’s Homewood campus.

Friday, May 13 will feature a symposium with talks from a slate of faculty experts in nanotechnology, oncology, engineering and medicine. Registration begins at 8:30 a.m. in Shriver Hall Auditorium.  A poster session begins at 1:30 p.m. upstairs in the Clipper Room showcasing research from INBT affiliated faculty laboratories across several Johns Hopkins University divisions. Past symposiums have attracted as many as 500 attendees and more than 100 research posters.

Keep checking INBT’s 2011 symposium page for updated information on speakers and more details on how to register and submit a poster title. The symposium and poster session are free for Johns Hopkins affiliated faculty, staff and students.

Keynote Speaker

Stephen B. Baylin is currently Deputy Director, Professor of Oncology and Medicine, Chief of the Cancer Biology Division and Director for Research, of The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins.For the last 20 years, Stephen Baylin has studied the role of epigenetic gene silencing in the initiation and progression of human cancer. He and his colleagues have fostered the concept that DNA hypermethylation of gene promoters, and associated transcriptional silencing, can serve as an alternative to mutations for producing loss of tumor suppressor gene function. They have described some of the classic genes involved, invented approaches to randomly screen the cancer genome for such genes and to demonstrate their functional role in cancer progression, helped begin unravel the molecular mechanisms responsible for the initiation and maintenance of the gene silencing, and worked to utilize all of their findings for translational purposes.  Baylin has authored or co-authored over 375 full-length publications on the above and other areas of cancer biology.

Stephen Baylin will present the keynote talk at the 2011 Johns Hopkins Nano-Bio Symposium

He has been a member of committees of the American Cancer Society and of National Institutes of Health, and his honors include a Research Career Development Award from NIH, the Edwin Astwood Lectureship of the Endocrine Society, the 2003 Jack Shultz Memorial Lecture in Genetics, Fox Chase  Cancer Center, The 2004 National Investigator of the Year Award from the National Cancer Institute SPORE program, the Jack Gibson Visiting Professorship, University of Hong Kong Queen Mary Hospital, Hong Kong, The 2004 2nd Annual Sydney E. Salmon Lectureship in Translational Research, Arizona Cancer Center, the 2005 Shubitz Cancer Research Prize from the University of Chicago, and he currently holds the Virginia and D.K. Ludwig Chair in Cancer Research at Johns Hopkins. Baylin is also recipient of the 2007 Woodward Visiting Professor, Memorial Sloan-Kettering Cancer Center, the 2008 Raffaele Tecce Memorial Lecture, Trento, Italy, the 2008 The David Workman Memorial Award (jointly with Peter A. Jones, Ph.D.) from the Samuel Waxman Foundation, and the 2009 Kirk A. Landon-AACR Prize for Basic Cancer Research, also shared with Peter A. Jones, the 14th NCI Alfred G. Knudson Award in Cancer Genetics, and, most recently, the Nakahara Memorial Lecture prize at the 2010 Princess Takematsu  Symposium. Currently, he leads, with Peter Jones, the Epigenetic Therapy Stand up to Cancer Team.

Additional confirmed speakers for the 2011 INBT Symposium include:

  • Martin Pomper is a professor at Johns Hopkins School of Medicine with a primary appointment in Radiology and secondary appointments in Oncology, Radiation Oncology, and Pharmacology and Molecular Sciences, as well as Environmental Health Sciences at the Johns Hopkins Bloomberg School of Public Health. Pomper co-directs Johns Hopkins Center of Cancer Nanotechnology Excellence (CCNE).
  • Anirban Maitra is a professor at Johns Hopkins School of Medicine with appointments in Pathology and Oncology at Sol Goldman Pancreatic Research Center and secondary appointments in Chemical and Biomolecular Engineering at the Whiting School of Engineering and the McKusick-Nathans Institute of Genetic Medicine. Maitra co-directs Johns Hopkins Cancer Nanotechnology Training Center and is a project director in the CCNE.
  • Jin Zhang is an associate professor at Solomon H. Snyder Department of Neuroscience at Johns Hopkins School of Medicine with primary appointments in Pharmacology and Molecular Sciences and secondary appointments in Neuroscience, Oncology, and Chemical and Biomolecular Engineering.
  • Hy Levitsky is a professor of Oncology, Medicine and Urology at the Johns Hopkins School of Medicine and the Scientific Director of the George Santos Bone Marrow Transplant Program. Levitsky is a project director at the Center of Cancer Nanotechnology Excellence (CCNE).
  • Gregory Longmore is a professor at the Washington University in St. Louis School of Medicine, Department of Medicine, Oncology Division, Molecular Oncology Section and the Department of Cell Biology and Physiology. Longmore is a project co-director at Johns Hopkins Physical Sciences-Oncology Center (PS-OC).
  • Denis Wirtz is the Theophilus H. Smoot Professor of Chemical and Biomolecular Engineering in the Whiting School of Engineering at Johns Hopkins University. Wirtz is associate director of INBT and director of the Johns Hopkins Physical Sciences-Oncology Center, also known as the Engineering in Oncology Center. He has a secondary appointment in Oncology at the Johns Hopkins School of Medicine.

Workshops

During the afternoon of May 12, INBT will hold four 2-hour hands-on laboratory workshops organized by faculty affiliated with INBT, PS-OC or CCNE. Workshop registration will be limited to 10 persons per session. Sessions will begin at 1 and 3:30 p.m. and will be held in the New Engineering Building. Workshop details, including any costs, are forthcoming.

Become a sponsor

If you or your organization would like to learn how to sponsor INBT’s annual symposium, please contact our director of corporate partnerships, Tom Fekete, at tmfeke@jhu.edu or call him at 410-516-8891. Sponsors enjoy reduced rates on symposium-related events and advertising in our annual Nano-Bio magazine/symposium program, among other benefits.

Media inquiries may be directed to Mary Spiro, science writer and media relations director for INBT, at mspiro@jhu.edu or 410-516-4802.

JHU Applied Physics Lab hosting 2nd Annual Nanomaterials Symposium

The Johns Hopkins Applied Physics Laboratory will host its 2nd Annual Nanomaterials Symposium on Monday, March 14 from 8:30 a.m. to 5 p.m. in the Kossiakoff Conference and Education Center, 11100 Johns Hopkins Road, Laurel, Md. 20723-6099. Come hear stimulating talks and network with speakers, attendees, and
sponsor panelists. Includes a special session for students on postdoctoraal and internship opportunities. Submit a poster for the poster session.

The symposium is FREE for students, but $25 for all others, and lunch is included.

Deadline to register is 5 p.m. March 8. Register online here.

Invited speakers include:

  • Jonah Erlebacher, Johns Hopkins University/INBT
  • Jason Benkoski, JHU Applied Physics Laboratory/INBT
  • Lourdes Salamanca-Riba, University of Maryland College Park
  • Hai-Quan Mao, Johns Hopkins University/INBT
  • Theodosia Gougousi, University of Maryland Balitmore County
  • Gary Rubloff, University of Maryland College Park
  • Brian Holloway, Defense Advanced Research Projects Agency

For additional information:

Johns Hopkins Applied Physics Lab

240-228-9166

INBT launches Johns Hopkins Center of Cancer Nanotechnology Excellence

Martin Pomper and Peter Searson will co-direct INBT’s new Center of Cancer Nanotechnology Excellence (Photo: Will Kirk/Homewood-JHU)

Faculty members associated with the Johns Hopkins Institute for NanoBioTechnology have received a $13.6 million five-year grant from the National Cancer Institute to establish a Center of Cancer Nanotechnology Excellence. The new Johns Hopkins center brings together a multidisciplinary team of scientists, engineers and physicians to develop nanotechnology-based diagnostic platforms and therapeutic strategies for comprehensive cancer care. Seventeen faculty members will be involved initially, with pilot projects adding more participants later.

The Johns Hopkins Center of Cancer Nanotechnology Excellence, which is part of the university’s Institute for NanoBioTechnology, is one of several NCI-supported centers launched through a funding opportunity started in 2005. According to the NCI, the program was established to create “multi-institutional hubs that integrate nanotechnology across the cancer research continuum to provide new solutions for the diagnosis and treatment of cancer.”

Peter Searson, who is the Joseph R. and Lynn C. Reynolds Professor of Materials Science and Engineering in the Whiting School of Engineering and director of the Institute for NanoBioTechnology, will serve as the center’s director. The co-director will be Martin Pomper, professor of radiology and oncology at the School of Medicine and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins.

“A unique feature of the center is the integration of research, education, training and outreach, and technology commercialization,” Searson said.

To move these new technologies toward use by physicians, a Cancer Nanomedicine Commercialization Working Group will be established and headed by John Fini, director of intellectual property for the university’s Homewood campus. This group will be responsible for managing and coordinating the translational process.

Another special feature of the center will be its Validation Core, led by Pomper, who is also associate director of the Johns Hopkins In Vivo Cellular and Molecular Imaging Center and director of the Johns Hopkins Small Animal Imaging Resource Program.

“Validation is about assuring that the experimental products and results we generate are on target and able to measure the biological effects for which they’re intended,” he said.

Searson and Pomper said the center will consist of four primary research projects.

One project will seek methods to screen bodily fluids such as blood or urine for indicators of cancer found outside of the genetic code, indicators called epigenetic markers. Led by Tza-Huei “Jeff” Wang, associate professor of mechanical engineering in the Whiting School of Engineering; Stephen Baylin, the Virginia and Daniel K. Ludwig Professor of Cancer Research in the School of Medicine; and James Herman, a professor of cancer biology in the School of Medicine, this project will use semiconductor nanocrystals, also known as quantum dots, and silica superparamagnetic particles to detect DNA methylation. Methylation adds a chemical group to the exterior of the DNA and is a biomarker frequently associated with cancer.

A second project, led by Anirban Maitra, associate professor of pathology and oncology at the School of Medicine and the Johns Hopkins Kimmel Cancer Center, will focus on curcumin, a substance found in the traditional Indian spice turmeric. In preclinical studies, curcumin has demonstrated anti-cancer properties but, because of its physical size, it is not readily taken up into the bloodstream or into tissues. Engineered curcumin nanoparticles, however, can more easily reach tumors arising in abdominal organs such as the pancreas, Maitra said. This team will try to determine whether nanocurcumin, combined with chemotherapeutic agents, could become a treatment for highly lethal cancers, such as pancreatic cancer.

Hyam Levitsky, professor of oncology at the Johns Hopkins Kimmel Cancer Center, will lead a third project, which will seek to use a noninvasive method to monitor the effectiveness of vaccines for cancer and infectious diseases.

A final project will build on the work of Justin Hanes and Craig Peacock, professors in the School of Medicine, to deliver therapies directly to small cell lung cancer tissue via mucus-penetrating nanoparticles.

All research efforts will be supported by a nanoparticle engineering core, led by Searson, which will make and characterize a variety of nanomaterials. Another core, centering on bioinformatics and data sharing, will be led by Rafael Irizarry, professor of biostatistics at the Johns Hopkins Bloomberg School of Public Health.

Johns Hopkins Institute for NanoBioTechnology

Sidney Kimmel Comprehensive Cancer Center

Johns Hopkins Researchers Appointed to Governor’s Task Force to Study Nanobiotechnology

Peter Searson

Steve Desiderio

Maryland Gov. Martin O’Malley has appointed Peter Searson and Steve Desiderio, two researchers from The Johns Hopkins University, to serve on a special task force to study the benefits of nanobiotechnology.

According to the governor’s office, the mission of the task force is “to study the benefits of nanobiotechnology including job creation, the development of lifesaving treatments, reductions in health care costs, the development of state-of-the-art electronics, medical equipment, chemical processes and other commercial products.”

Nanotechnology involves the application of materials and devices at the scale of just a few atoms in diameter. Nanobiotechnology attempts to apply these tiny technologies to medicine and basic science.

Searson is the Joseph R. and Lynn C. Reynolds Professor of Engineering in the university’s Whiting School of Engineering. He is a professor in the Department of Materials Science and Engineering, and he directs the Johns Hopkins Institute for NanoBioTechnology. He received his Ph.D. from the University of Manchester in England and was a postdoctoral associate at the Massachusetts Institute of Technology. He lives in Baltimore.

Desiderio is director of the Institute of Basic Biomedical Sciences, director of the Immunobiology Program at the Institute for Cell Engineering and a professor of Molecular Biology and Genetics at the Johns Hopkins University School of Medicine. Desiderio earned his M.D. and a Ph.D. from the Johns Hopkins University School of Medicine and was a postdoctoral fellow at the Massachusetts Institute of Technology. Desiderio also lives in Baltimore.

Both Searson and Desiderio are involved with research related to nanobiotechnology. Searson’s interests include nanoscience, biophysics and bioengineering. He led the launch of the Institute for Nanobiotechnology, which was established in 2006 as a cross-divisional center with research interests in the basic sciences, engineering, medicine and public health.

Desiderio’s research focuses on the immune system: how immune cells are able to recognize a diverse number of pathogens and respond to environmental cues. He studies the molecular and genetic mechanisms underlying the development of the immune system. In 2007, Desiderio was appointed by O’Malley to the Maryland Life Sciences Advisory Board.

The nanobiotechnology task force will be chaired by state Sen. Jennie M. Forehand and Del. Susan C. Lee. In addition to Searson and Desiderio, task force members include Nariman Farvadin, Peter Swaan, Esther H. Chang, Lisbeth Pettengill, Patrick Y. Lu and Lawrence Tamarkin.

Along with examining the scientific and medical benefits of nanobiotechnology, the task force members expect to look at the economic impact that the development of such technologies might have on the state of Maryland, including the creation of jobs.

The governor’s office also stated that the group will study the “generation of revenue for the state and improvements to the quality of life for the state’s citizens and the state’s role in supporting Maryland’s leadership in nanobiotechnology, including: promoting public-private partnerships; assisting companies in technology transfers, including from research to commercial product; promoting research; protecting intellectual property; offering appropriate financial incentives; including tax credits; and capturing and leveraging federal funds for both public and private ventures; and make recommendations regarding actions that the state should take to promote the growth of the nanobiotechnology industries in the state.”

Lights! Camera! Science!

 

INBT Web Director Martin Rietveld works on protocol video with PhD student Yu-Ja Huang. (Photo:MSpiro)

Everything about movie making seems so glamorous. From beautiful stars to special effects, making films might appear magical. But actually, when you break it down, shooting a film is not unlike performing experiments in a lab. And, just as reading the script would be far less entertaining as seeing a film, reading a protocol might be confusing until the steps were performed in real life.

That’s the philosophy behind a new effort at Johns Hopkins Institute for NanoBioTechnology: produce short films describing recently published research and the protocols that go with them. The movies are produced collaboratively with INBT’s science writer Mary Spiro, INBT’s Animation Studio director Martin Rietveld, and the scientists and engineers involved.

The INBT Animation Studio already has several research-oriented films to its credit. The animation skills of Rietveld and his student crew have taken us inside a lipid bilayer and carried us along a fiber of collagen. INBT also has produced several video news releases using the talent of students in the annual science communication course.

Recently, however, INBT produced its first film describing a protocol from Nature Methods. Investigators Bridget Wildt, a PhD in materials science and engineering, Peter Searson, Reynolds Professor of Materials Science and Engineering, and Denis Wirtz, Smoot Professor of Chemical and Biomolecular Engineering, served as technical consultants for the production. The research was part of Johns Hopkins Engineering in Oncology Center, of which Wirtz is the director.

Materials science and engineering PhD candidate Yu-Ja Huang performs each step in assembling the Hopkins team’s device and demonstrates how to conduct programmed cell detachment experiments. “Studying cell detachment at the subcellular level is critical to understanding the way cancer cells metastasize,” Searson said. “Development of scientific methods to study cell detachment may guide us to prevent, limit or slow down the deadly spreading of cancer cells.”

Using a draft script developed by Wildt and Searson, Spiro simplified the text further for narrator, materials science and engineering PhD candidate Andrew Wong. Rietveld recorded Huang as he performed the protocol and refined the script further during filming. Viewing the final cut, Wong was able to read the script in a conversational and friendly tone.

You can watch the version of this new protocol video on INBT’s YouTube channel. The film may never earn an Academy Award, but we hope it will help specialists, and even the general public, to understand this unusual and complex procedure.

Related Links:

Check our INBT’s channel on YouTube.

Engineering in Oncology Center

Story by Mary Spiro

Poster presenters needed for symposium on environmental, health impacts of nanotech

2009 INBT Poster Session (Photo: Jon Christofersen)

Poster titles are now being accepted for Johns Hopkins Institute for NanoBioTechnology’s fourth annual symposium, “Environmental and Health Impacts of Engineered Nanomaterials” set for Thursday, April 29, at the Bloomberg School of Public Health. Researchers from across the university, from government and industry, and from other universities are invited to submit posters by the deadline of April 22.

All students, faculty and staff affiliated with any Johns Hopkins campus or school may attend the symposium for free. Students from UMBC and Morgan State University may also attend at no cost.

This year’s symposium brings together faculty experts engaged in various aspects of nanotechnology risk assessment and management research. Jonathan Links, an INBT-affiliated professor in the Department of Environmental Health Sciences at the Bloomberg School, assembled the slate of speakers from across four divisions of the university.

Links said that this diversity reflects the multidisciplinary approach needed to effectively address questions of how nanomaterials move through and interact with the environment, and how they may impact biological organisms, including humans. Links added that despite some concerted efforts to assess risk, many questions remain unanswered about how engineered nanomaterials and nanoparticles impact human health and the environment.

“Without these data, we are flying blind. But when risk assessment is performed in tandem with research into beneficial applications, it helps researchers make better decisions about how nanotechnology is used in the future,” Links said.

Along with Links, professors from the Bloomberg School presenting talks at the symposium include Ellen Silbergeld, of Environmental Health Sciences, and Patrick Breysse, of Environmental Health Engineering and Environmental Health Sciences. William P. Ball, a professor in the Whiting School of Engineering’s Department of Geography and Environmental Engineering; Justin Hanes, a professor in the School of Medicine’s Department of Ophthalmology, with joint appointments in the Whiting School’s Department of Chemical and Biomolecular Engineering and the Bloomberg School’s Department of Environmental Health Sciences; and Howard Fairbrother, a professor in the Krieger School of Arts and Sciences’ Department of Chemistry, will talk about the transport of nanomaterials through environmental and biological systems, as well as the unusual properties of manufactured nanomaterials.

Tomas Guilarte, recently appointed chair of the Department of Environmental Health Sciences at Columbia University’s Mailman School of Public Health and a former professor at the Bloomberg School, will provide a presentation on neurotoxicity of nanoparticles. Ronald White, an associate scientist and deputy director of the Bloomberg School’s Risk Sciences and Public Policy Institute, will discuss policy implications based on risk assessment.

Symposium talks will be from 8:30 a.m. until noon in Sheldon Hall (W1214), and a poster session, with prizes for top presenters, will be held from 1:30 to 3 p.m. in Feinstone Hall (E2030).

To register for the symposium or to display a poster, click here.

For more information about INBT’s fourth annual symposium, click here.

Story by Mary Spiro

Environmental, health impacts of engineered nanomaterials theme of INBT’s annual symposium

By 2015, the National Science Foundation reports that the nanotechnology industry could be worth as much as $1 trillion. Nanomaterials have many beneficial applications for industry, medicine and basic scientific research. However, because nanomaterials are just a few atoms in size, they also may pose potential risks for human health and the environment.

Cross-sectional autoradiograms of rodent brains showing (A) control physiological state; and (B) and (C) showing distribution of brain injury from an injected neurotoxicant. Red areas indicate the highest concentrations of a biomarker that identifies brain areas that are damaged by the neurotoxicant. (Guilarte Lab/JHU)

Cross-sectional autoradiograms of rodent brains showing (A) control physiological state; and (B) and (C) showing distribution of brain injury from an injected neurotoxicant. Red areas indicate the highest concentrations of a biomarker that identifies brain areas that are damaged by the neurotoxicant. (Guilarte Lab/JHU)

To increase awareness of Hopkins’ research in this emerging area of investigation, the theme for the fourth annual symposium hosted by Johns Hopkins Institute for NanoBioTechnology (INBT) will be environmental and health impacts of engineered nanomaterials. INBT’s symposium will be held Thursday, April 29, from 8 a.m. to 3 p.m. at the university’s Bloomberg School of Public Health in Baltimore, Md.

Morning talks in Sheldon Hall by eight Hopkins faculty experts will discuss neurotoxicity, exposure assessment, manufacture and characterization of nanomaterials, policy implications and many other topics. In the afternoon, a poster session will be held in Feinstone Hall featuring nanobiotechnology research from across the university’s divisions.

INBT is seeking corporate sponsorships for the symposium. Interested parties should contact Thomas Fekete, INBT’s director of corporate partnerships at tmfeke@jhu.edu or 410-516-8891.

Media inquiries should be directed to Mary Spiro, INBT’s science writer and media relations director, at mspiro@jhu.edu or 410-516-4802.

A call for posters announcement will be made at a later date.

More:

INBT, EOC directors named AAAS 2009 Fellows

The Johns Hopkins Whiting School of Engineering faculty members who direct the Institute for NanoBioTechnology and Engineering in Oncology Center both have been awarded the distinction of AAAS Fellow. Election as a Fellow is an honor bestowed upon AAAS members by their peers.

Peter Searson, INBT director. Photo by Will Kirk/JHU

Peter Searson, INBT director. Photo by Will Kirk/JHU

Denis Wirtz, EOC director. Photo by Will Kirk/JHU

Denis Wirtz, EOC director. Photo by Will Kirk/JHU

Peter C. Searson, the Joseph R. and Lynn C. Reynolds Professor of Materials Science and Engineering, was named for distinguished contributions to the field of surface chemistry and nanoscience. His research interests include surface and molecular engineering, and semiconductor quantum dots.

Searson directs the interdivisional Institute for NanoBioTechnology launched in May 2006, which brings together researchers from medicine, engineering, the sciences, and public health to create new knowledge and develop new technologies to revolutionize health care and medicine. INBT currently has more than 190 affiliated faculty members. Searson has secondary appointments in the Krieger School of Arts and Sciences Department of Physics and Astronomy and the Johns Hopkins School of Medicine Department of Oncology.

Denis Wirtz, the Theophilus H. Smoot Professor of Chemical and Biomolecular Engineering, was elected for his contributions to cell micromechanics and cell adhesion. He also was distinguished for his development and application for particle tracking methods to probe the micromechanical properties of living cells in normal conditions and disease state. Wirtz studies the biophysical properties of healthy and diseased cells, including interactions between adjacent cells and the role of cellular architecture on nuclear shape and gene expression.

Wirtz directs the newly formed Johns Hopkins Engineering in Oncology Center. The EOC is a Physical Sciences in Oncology program center of the National Cancer Institute launched in October 2009 with a $14.8 million grant from the National Institutes of Health. EOC brings together experts in cancer biology, molecular and cellular biophysics, applied mathematics, materials science, and physics to study and model cellular mobility and the assorted biophysical forces involved in the spread of cancer. Wirtz also serves as co-director of the Institute for NanoBioTechnology and has a joint appointment in the Johns Hopkins School of Medicine Department of Oncology.

A total of seven Johns Hopkins faculty members were elected to AAAS this year. Read about all of them in a Johns Hopkins University press release listed in the links below.

This year 531 members have been awarded this honor by AAAS because of their scientifically or socially distinguished efforts to advance science or its applications. New Fellows will be presented with an official certificate and a gold and blue (representing science and engineering, respectively) rosette pin on Feb. 20 at the AAAS Fellows Forum during the 2010 AAAS Annual Meeting in San Diego.  AAAS Fellows were announced in the AAAS News & Notes section of the journal Science on Dec. 18,  2009.

Story by Mary Spiro with materials provided by AAAS.

Seven Johns Hopkins Researchers Named 2009 AAAS Fellows

Searson Group Lab page

Wirtz Group Lab page

Johns Hopkins Institute for NanoBioTechnology

Whiting School of Engineering