Pluripotent stem cells hold key to blood vessel formation

Pluripotent stem cells, those cells capable of transforming into any type of tissue in the human body, hold the key to one of science’s biggest challenges: the formation of new blood vessels.

Researchers in the laboratory of Sharon Gerecht, associate professor of chemical and biomolecular engineering in the Whiting School of Engineering at Johns Hopkins University, have demonstrated a method that causes these powerful cells to form a fresh network of blood vessels when transplanted in mice. Shawna Williams, writer at the Johns Hopkins School of Medicine, reports here on this new research, which was published online this week in the Proceedings of the National Academy of Sciences. You can find the article here.

Shown are lab-grown human blood vessel networks (red) incorporating into and around mouse networks (green). (Gerecht Lab/PNAS)

Shown are lab-grown human blood vessel networks (red) incorporating into and around mouse networks (green). (Gerecht Lab/PNAS)

Here’s a comment from Gerecht, who is affiliated with both Johns Hopkins Physical Sciences–Oncology Center and Institute for NanoBioTechnology:

“In demonstrating the ability to rebuild a microvascular bed in a clinically relevant manner, we have made an important step toward the construction of blood vessels for therapeutic use … Our findings could yield more effective treatments for patients afflicted with burns, diabetic complications and other conditions in which vasculature function is compromised.”

The Gerecht lab, in collaboration with researchers at the School of Medicine, has been working on this puzzle for some time. One important stride in this current work is that the vessels are forming and persisting in a living animal and not just in a culture in a flask.

Says lead author and doctoral student in biomedical engineering, Sravanti Kusuma:

“That these vessels survive and function inside a living animal is a crucial step in getting them to medical application.”

You can read about some of the Gerecht lab’s previous findings in this particular pursuit in the articles listed below:

Engineers Coax Stem Cells to Diversify 

Research Seeks to Turn Stem Cells into Blood Vessels

 

Spring nano-bio mini-symposium set for April 3

Catch up on the latest research happening in Johns Hopkins University labs working in nanobiotechnology, the physics of cancer and cancer nanotech at INBT’s spring mini-symposium Wednesday, April 3 from 9 a.m. to 1 p.m. in Leverings’s Great Hall on the Homewood campus.

AT AT GLANCE- INBT new signSMALL

Mini-symposiums are organized in the spring and fall by student leaders in the Johns Hopkins Institute for NanoBioTechnology, the Engineering in Oncology Center and the Center of Cancer Nanotechnology Excellence. They are a means of showcasing current work, learning from guest speakers and facilitating communication and collaboration among affiliated laboratories. This event is open to the entire Johns Hopkins Community. Save the date!

The agenda is as follows:

  • 9:00 am ~ 9:10 am Welcome speech Denis Wirtz, PhD, Director of Johns Hopkins Physical Science Oncology Center (PS-OC)
  • 9:10 am ~ 9:40 am “Role of ion channels and aquaporins in cancer cell migration in confined microenvironments” Kimberly M. Stroka, PhD, Postdoc fellow (PS-OC) Department of Chemical and Biomolecular Engineering, Johns Hopkins University
  • 9:40 am ~ 10:10 am “TBD” Helena Zec, Graduate student (CCNE) Department of Biomedical Engineering, Johns Hopkins University
  • 10:10 am ~ 10:40 am “Single-cell protein profiling to study cancer cell heterogeneity” Jonathan Chen, Graduate student (PS-OC) Department of Biomedical Engineering, Yale University
  • 10:40 am ~ 11:30 am “Synthetic cell biology: total synthesis of cellular functions” Takanari Inoue, PhD, Assistant professor Department of Cell Biology, Johns Hopkins University School of Medicine
  • 11:30 am ~ 11:40 am Coffee Break
  • 11:40 am ~ 12:10 pm “TBD” Yu-Ja Huang, Graduate student (PS-OC) Department of Materials Science and Engineering, Johns Hopkins University
  • 12:10 pm ~ 1:00 pm “Infections, Chronic Inflammation, and Prostate Cancer” Karen Sandell Sfanos, PhD, Assistant professor Department of Pathology, Johns Hopkins University School of Medicine
  • 1:00 pm ~ 1:30 pm “Development of CEST liposomes for monitoring nanoparticle-based cancer therapies using MRI” Tao Yu, Graduate student (CCNE) Department of Biomedical Engineering, Johns Hopkins University

INBT Spring mini-symposium flyer

Siebel scholars demonstrate INBT’s multidisciplinary advantage

Siebel scholar Laura Ensign. Photo by Marty Katz.

Four of the five recently named Johns Hopkins University graduate students who were listed among the 2013 Siebel Scholars are affiliated with Johns Hopkins Institute for NanoBioTechnology laboratories. Three of the four were also part of INBT’s Nanobio IGERT, or Integrative Graduate Education Research Traineeship, a National Science Foundation funded program. The Siebel Scholars program recognizes the most talented students at the world’s leading graduate schools of business, bioengineering, and computer science.

INBT affiliated winners include Laura Ensign, Mustapha Jamal, Garrett Jenkinson and Yi Zhang. Ensign, Jamal and Jenkinson were INBT IGERT fellows. All note that their involvement with INBT to one degree or another has played a role in their academic success at Hopkins.

Laura Ensign, in the Department of Chemical and BioMolecular Engineering, works in the laboratory of Justin Hanes, who is director of the Center for Nanomedicine and investigator with the Center of Cancer Nanotechnology Excellence (CCNE). Ensign’s research involves understanding the mucus barrier in the female reproductive tract and how it protects and also inhibits the delivery of drugs to this part of the body. Using specially engineered mucus penetrating nanoparticles designed in the Hanes labs, she is working on more effective drug delivery systems. Ensign is listed as an inventor on three patents that have been licensed to private industry.

“As an engineer, the multidisciplinary nature of INBT has allowed me to do research that has the potential to help patients in the clinic,” Ensign said. Furthermore, Ensign noted that having two advisors, a requirement for INBT’s IGERT program, played an important role in her graduate work and discoveries. In addition to being advised by Hanes, Ensign also was mentored by Richard Cone, professor in the Department of Biophysics in the Krieger School of Arts and Sciences. “The trajectory of my research has been greatly influenced by having two advisers with different backgrounds. My research has included engineering and formulation aspects, as well as biological and translational aspects, resulting in higher impact results with broader implications. “

Siebel scholar Mustapha Jamal

Mustapha Jamal, also in the Department of Chemical and Biomolecular Engineering, worked in the laboratory of associate professor David Gracias. Jamal has developed self-assembling structures that provide a framework for 3D tissue culture. In addition, these self-assembling structures let him study how geometry affects cell behavior. Jamal is a co-inventor on a patent application in connection with this research.

“Working in a multidiscplinary lab has helped me engineer miniaturized 3D cell culture platforms utilizing techniques from seemingly disparate research areas: semiconductor processing and tissue engineering,” Jamal said. “With a bit of creativity, this diverse skill set has proven useful in forging exciting and fruitful collaborations and should serve me well for years to come. From the annual INBT Symposium to the courses and workshops, I have shared my own research with the community and engaged in academic discussions that have helped me keep on top of research conducted here at Hopkins and abroad.”

Siebel scholar Garrett Jenkinson learning wet lab skills during INBT’s nanobio bootcamp. Photo by Mary Spiro.

Mathematics is the tool that W. Garrett Jenkinson uses in his research in the Complex Systems Science Laboratory of John Goutsias, professor of electrical and computer engineering. Jenkinson’s work can be applied to such real-life problems as how infections spread through a population via social interaction or how processes occur inside the cell, both of which can help inform the development of drugs to fight disease.

“The Complex Systems Science Laboratory takes the INBT spirit of interdisciplinary research to heart. The lab focuses on rigorous mathematical formulations that will simultaneously advance as many branches of science and engineering as possible,” Jenkinson said. “My graduate work has allowed me to follow my mathematical interests toward whatever application they might lead. In my tenure at Hopkins, I have published papers on a diverse array of topics including biochemical reaction networks, epidemiology, neurobiology, ecology, thermodynamics, unmanned automated vehicles, evolutionary game theory, pharmacokinetics, and social networks.

Through the IGERT program, Jenkinson said, INBT “trained me in fields that an electrical and computer engineer might otherwise find foreign, such as biology, nanotechnology, and wet lab techniques. Furthermore, the INBT has fostered relationships with my peers from diverse scientific backgrounds, with whom I have collaborated on multiple occasions to lend or receive advice in scientific matters that required expertise in multiple fields. I am excited to be joining the Siebel Scholars program which facilitates relationships across universities in the same way the INBT fosters these relationships across departments at Johns Hopkins University.”

Siebel scholar Yi Zhang. Photo by Mary Spiro.

Yi Zhang conducts his research in the lab of Jeff Tza-Huei Wang, an associate professor of mechanical engineering, biomedical engineering and oncology and also a project leader in the Center of Cancer Nanotechnology Excellence. Zhang’s work developing micro- and nanoscale molecular techniques to help diagnose cancer and infectious diseases has supported one of the core research goals of the CCNE. He is listed as an inventor on four patent applications, one of which has been licensed by a biotechnology company.

Said Zhang, “Being associated with an INBT affiliated laboratory offers me ample opportunities to collaborate with researchers in various fields and get help from my fellow students. Biomedical engineering is multidisciplinary in nature. My research focuses on bridging the gap between medical science and engineering, and my thesis is committed to improving molecular diagnostics using advanced nanotechnology. An integrated center like CCNE presents a new research paradigm by bringing together all necessary expertise from various fields to tackle one big problem in an extremely efficient way. It has definitely changed my view of conducting translational research.”

According to the organization’s website, Siebel Scholars and are chosen by the dean of their respective schools on the basis of outstanding academic achievement and demonstrated leadership. On average, Siebel Scholars rank in the top 5 percent of their class, many within the top 1 percent. The merit-based program provides $35,000 to each student for use in his or her final year of graduate studies.

The Siebel Scholars program was established in 2000 by the Siebel Foundation through a grant of more than $45 million to Carnegie Mellon University; Harvard University; The Johns Hopkins University; Massachusetts Institute of Technology; Northwestern University; Stanford University; Tsinghua University; University of California, Berkeley; University of California, San Diego; University of Chicago; University of Illinois at Urbana-Champaign; and University of Pennsylvania. Each year, five graduate students from each of the 17 partner institutions are honored as Siebel Scholars and receive a $35,000 award for their final year of studies.

Established in 2006, the Institute for NanoBioTechnology at Johns Hopkins brings together 223 researchers from every division of the University to create new knowledge and new technologies at the interface of nanoscience and medicine.

 

Save the date: fall mini-symposium set for Oct. 24

Graduate students and post doctoral fellows from the Johns Hopkins Institute for NanoBioTechnology, Center of Cancer Nanotechnology Excellence and Physical Science-Oncology Center will host a mini-symposium highlighting some of the current investigations occurring in these research entities. The symposium will include short talks from six to eight researchers and will be held on Wednesday, Oct. 24 from 9 a.m. to noon at a Homewood campus location to be determined. Check back for location and agenda.

View the agendas from previous INBT/CCNE/PSOC mini-symposiums  at the links below:

Spring 2012

Fall 2011

Spring 2011

Meet INBT’s summer interns, already digging into their research

Research does not take a holiday during the summer at Johns Hopkins University in Baltimore, Md. In fact, it ramps up with the addition of many new faces from across the country.

The Johns Hopkins Institute for NanoBioTechnology summer research interns have arrived and are already busy at work in various laboratories. This year’s group is the largest the institute has ever hosted, with 17 undergraduates from universities nationwide.

Of the total, three students are affiliated with the Center of Cancer Nanotechnology Excellence and four are affiliated with the Physical Sciences-Oncology Center. The remaining 10 are part of the National Science Foundation Research Experience for Undergraduates program. All are hosted through INBT, which serves as a hub for their academic and social activities.

INBT summer interns conduct 10 weeks of research in a laboratory either on the Homewood or the medical campus of the University. At the end of that time, students have learned how to work in a multidisciplinary team and how to manage a short term research project.  They also discover if research is a pathway they want to pursue after earning their bachelor’s degrees.

In August, interns from many of the science, medicine, engineering and public health summer programs will gather for a  poster session to be held on August 2 at 3 p.m. in Turner Concourse. The poster session will allow students to show off the results of their their work.

This year’s INBT/PS-OC/CCNE interns include:

At the Whiting School of Engineering…

Amani Alkayyali from Wayne State University is an REU student in the laboratory of Honggang Cui assistant professor in the Department of Chemical and Biomolecular Engineering. Also in the Cui lab are CCNE intern Matthew Fong from the University of California, Berkeley and Michelle LaComb, an REU student from Rice University.

Sharon Gerecht, assistant professor in the Department of Chemical and Biomolecular engineering, is hosting three interns. Josh Porterfield of Cornell University and Carolyn Zhang from the University of California, San Diego are both PS-OC interns, and Bria Macklin of Howard University is an REU intern.

Jacqueline Carozza of Cornell University is a PS-OC student working in the lab of Denis Wirtz, professor in the Department Chemical and Biomolecular Engineering. Cassandra Loren from Oregon State University is a PS-OC intern also working in the Wirtz lab.

Eric Do from the University of Washington is an REU working in the lab of assistant professor Margarita Herrara-Alonso in the Department of Materials Science and Engineering.

Olivia Hentz from Cornell is an REU student working in the lab of Jonah Erlebacher, professor in the Department of Materials Science and Engineering.

Justin Samorajski from the University of Dallas is a returning summer intern, once again working in the materials science and engineering lab of professor Peter Searson as part of the CCNE.

At the School of Medicine…

Lauren Lee of Cornell University is an REU working in the lab of biomedical engineering lab of associate professor Hai-Quan Mao.

Albert Lu from the University of California Berkeley is a CCNE intern working in the biomedical engineering lab of associate professor Jeff Wang.

Bianca Lascano from Norfolk State University is an REU in assistant professor Jordan Green’s biomedical engineering lab.

Charlie Nusbaum of the Richard Stockton College is an REU intern in the radiation oncology lab of assistant professor Robert Ivkov.

At the Krieger School of Arts and Sciences…

Anthony Loder of Rowan University is an REU working in the biology lab of assistant professor Xin Chen.

Daniel McClelland is also REU from Bethany College works in the chemistry laboratory of professor Howard Fairbrother.