This a method to measure the local and global micromechanical properties of the cytoplasm of single living cells in their physiological milieu and subjected to external stimuli. By tracking spontaneous, Brownian movements of individual nanoparticles of diameter ? 100 nm distributed within the cell with high spatial and temporal resolutions, the local viscoelastic properties of the intracellular milieu can be measured in different locations within the cell.

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