Light/Electron Microscopy

  • Integrated Imaging Center
    The Integrated Imaging Center has the necessary instrumentation (hardware and software) required for most basic and advanced LM/EM techniques. Equipment includes:

    • FEI Tecnai 12 TWIN equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS Megaview III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI; and NIH SIG award # 1S10RR023454-01, McCaffery PI)
    • Philips EM 420 TEM equipped with SIS Megaview III CCD digital camera (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
    • FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)
    • Anatech Hummer 6.2 sputter coater
    • Tousimis Model 795 critical point dryer
    • 2 LEICA Ultracut UCT Ultramicrotomes/FCS Cryostages (acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)
    • Zeiss LSM 510 Meta Confocal with motorized stages and 405nm laser (acquired in partwith funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
    • Zeiss LSM 510 VIS Confocal with Confocor 3 FCS (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
    • 3-I Marianas Live Cell Imaging Workstation equipped with dual Cascade II 512 EM cameras for simultaneous 2-channel acquistion and TIRF (acquired with funds from NIH SIG award #1S10RR022588-01, McCaffery PI)
    • Zeiss Axiovert inverted LM equipped with excitation and emission filter wheels; Cooke and I-Pentamax 512 digital cameras;also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
    • Zeiss Axioplan upright LM equipped with a CoolSnap digital monochrome camera (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI; and NIH R01GM060979-06, Wendland PI)
    • Typhoon 9410 Variable Mode Imager capable of storage phosphor, chemiluminescence, and fluorescence (acquired with funds from NIH SIG award 1S10RR021023-01, McCaffery PI)
    • Avestin EmulsiFlex C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
    • Eppendorf Microijector 5170/Micromanipulator 5242
    • Bioptech FCS-2 Environmental Stage (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
    • Instec 0°-60°C stage (peltier modulated) (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
    • Quantum Snap! Servers-3.0 Tbytes (acquired in part with funds from NIH SIG awards 1S10RR021023-01, McCaffery PI; and 1S10RR019409-01, McCaffery PI )
    • Remote SGI, Sun, and Mac/PC post-acquistion image processing/analysis workstations
    • AGFA T2500 Multi-purpose/Multi-format color film scanner (for both reflective and transmitted scanning with up to 4000 dpi resolution)

    Contact:
     Michael McCaffery, Director
    (410) 516-3321 (Office)
    (410) 516-7324 (Lab)
    jmccaffery@jhu.edu
    http://www.jhu.edu/iic

  • School of Medicine Microscope Facility
    The facility assists investigators with research requiring light and electron microscopy and electronic imaging. Equipment includes: LEO Field Emission SEM; OLYMPUS IX81 Inverted Microscope; Perkin Elmer UltraVIEW Spinning Disk Confocal Microscopes; PHILIPS BioTwin CM120 TEM; ZEISS/eppendorf Microinjection Station; ZEISS Multiple Fluorescence Imaging; Leica cryostat; Zeiss 510 Meta confocal microscope; Hitachi 7600 TEM; Olympus TIRF station; Olympus TIRF station 2Contact:
    Prof. Douglas B. Murphy, Director
    Michael Delannoy, Associate Director
    (410) 955-4536 (Office)
    (410) 955-1365 (Lab)
    http://www.hopkinsmedicine.org/micfac/
  • Electron Microbeam Analytical Facility
    Atomic resolution and material imaging, nanometer and micrometer-scale chemical analysis. The instruments include: a FEI CM300 with FEG and GIF, a Philips 420ST TEM, and a JEOL 8600 SuperprobeContact:
    Ken Livi, Sr. Electron Microscope Research Scientist
    (410) 516-8342
    Fax (410) 516-7933
    klivi@jhu.edu
  • Scanning Electron Microbeam Analytical Laboratory
    EOL 6700 FEM High Resolution SEM with EDSContact:
    Mark Koontz
    (410) 516-5335
    mkoontz@jhu.edu
    http://www.jhu.edu/~matsci/facilities