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Integrated Imaging Center
The Integrated Imaging Center has the necessary instrumentation (hardware and software) required for most basic and advanced LM/EM techniques. Equipment includes:
- FEI Tecnai 12 TWIN equipped with 16 bit 2K x2K FEI Eagle bottom mount camera; and SIS Megaview III wide-angle camera (acquired with funds from NSF DBI award # 0099706, McCaffery PI; and NIH SIG award # 1S10RR023454-01, McCaffery PI)
- Philips EM 420 TEM equipped with SIS Megaview III CCD digital camera (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
- FEI Quanta 200 Environmental SEM (acquired with funds from HHMI undergraduate education grant)
- Anatech Hummer 6.2 sputter coater
- Tousimis Model 795 critical point dryer
- 2 LEICA Ultracut UCT Ultramicrotomes/FCS Cryostages (acquired in part with funds from NSF DBI award # 0099706, McCaffery PI)
- Zeiss LSM 510 Meta Confocal with motorized stages and 405nm laser (acquired in partwith funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
- Zeiss LSM 510 VIS Confocal with Confocor 3 FCS (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
- 3-I Marianas Live Cell Imaging Workstation equipped with dual Cascade II 512 EM cameras for simultaneous 2-channel acquistion and TIRF (acquired with funds from NIH SIG award #1S10RR022588-01, McCaffery PI)
- Zeiss Axiovert inverted LM equipped with excitation and emission filter wheels; Cooke and I-Pentamax 512 digital cameras;also equipped for microinjection and micromanipulation (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
- Zeiss Axioplan upright LM equipped with a CoolSnap digital monochrome camera (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI; and NIH R01GM060979-06, Wendland PI)
- Typhoon 9410 Variable Mode Imager capable of storage phosphor, chemiluminescence, and fluorescence (acquired with funds from NIH SIG award 1S10RR021023-01, McCaffery PI)
- Avestin EmulsiFlex C3 (acquired in part with funds from NIH R01GM060979-06, Wendland PI)
- Eppendorf Microijector 5170/Micromanipulator 5242
- Bioptech FCS-2 Environmental Stage (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
- Instec 0°-60°C stage (peltier modulated) (acquired in part with funds from NIH SIG award #1S10RR019409-01, McCaffery PI)
- Quantum Snap! Servers-3.0 Tbytes (acquired in part with funds from NIH SIG awards 1S10RR021023-01, McCaffery PI; and 1S10RR019409-01, McCaffery PI )
- Remote SGI, Sun, and Mac/PC post-acquistion image processing/analysis workstations
- AGFA T2500 Multi-purpose/Multi-format color film scanner (for both reflective and transmitted scanning with up to 4000 dpi resolution)
Contact:
Michael McCaffery, Director
(410) 516-3321 (Office)
(410) 516-7324 (Lab)
jmccaffery@jhu.edu
http://www.jhu.edu/iic - School of Medicine Microscope Facility
The facility assists investigators with research requiring light and electron microscopy and electronic imaging. Equipment includes: LEO Field Emission SEM; OLYMPUS IX81 Inverted Microscope; Perkin Elmer UltraVIEW Spinning Disk Confocal Microscopes; PHILIPS BioTwin CM120 TEM; ZEISS/eppendorf Microinjection Station; ZEISS Multiple Fluorescence Imaging; Leica cryostat; Zeiss 510 Meta confocal microscope; Hitachi 7600 TEM; Olympus TIRF station; Olympus TIRF station 2Contact:
Prof. Douglas B. Murphy, Director
Michael Delannoy, Associate Director
(410) 955-4536 (Office)
(410) 955-1365 (Lab)
http://www.hopkinsmedicine.org/micfac/ - Electron Microbeam Analytical Facility
Atomic resolution and material imaging, nanometer and micrometer-scale chemical analysis. The instruments include: a FEI CM300 with FEG and GIF, a Philips 420ST TEM, and a JEOL 8600 SuperprobeContact:
Ken Livi, Sr. Electron Microscope Research Scientist
(410) 516-8342
Fax (410) 516-7933
klivi@jhu.edu - Scanning Electron Microbeam Analytical Laboratory
EOL 6700 FEM High Resolution SEM with EDSContact:
Mark Koontz
(410) 516-5335
mkoontz@jhu.edu
http://www.jhu.edu/~matsci/facilities
Facilities
Light / Electron Microscopy
X-Ray
- Surface Analysis Laboratory
PHI 5100 x-ray photoelectron spectrometer, PHI 610 scanning Auger electron spectrometerContact:
Mark Koontz
410 516-5335
mkoontz@jhu.edu
http://www.jhu.edu/~matsci/facilities/
- JHU Small Molecule Crystallography
Xcalibur3 X-ray diffractometer; 3kW sealed-tube generator, running Mo radiation.
Contact:
xray@jhu.edu - Macromolecular Crystallography Center
Rigaku generator; R-axis Vi++ image plate detector.
Contact:
Joel Schildbach, Director
(410) 516-0176 (Office)
joel@jhu.edu
website
Cell Culture
- The Cell Center of the Genetic Resources Core Facility
The Cell Center facilitates basic scientific research by providing expertise and service in all aspects of mammalian cell culture.
Contact:
GRCF Cell Center
(410) 955-3320
FAX: (410) 614-764
Genotyping
- The Fragment Analysis Facility of the Genetic Resources Core Facility
The Fragment Analysis Facility provides technical assistance with genotyping. Services includes polymorphism analysis, microsatellite interpretation and isolation of genomic DNA.
Contact:
(410) 614-3830
FAX (410) 614-3148
Proteometrics
- The Synthesis and Sequencing Facility
The facility provides services in oligonucleotide synthesis and purification, peptide synthesis and purification, N-terminal sequence analysis and DNA sequencing. The equipment includes: a MALDI-TOF Voyager DE-STR (Applied Biosystems) and an electrospray ionization mass spectrometer QStar/Pulsar (Applied Biosystems/MDS Sciex).
Contact:
Peter L. Pedersen, Director
Jodie Franklin, Manager
(410) 955-2739
http://biolchem.bs.jhmi.edu/facilities/ - AB Mass Spectrometry/Proteomic Facility
The Mass Spectrometry Facility currently has three mass spectrometers, a Voyager DE-STR (Applied Biosystems), QSTAR/Pulsar (Applied Biosystems/MDS Sciex) and LCQ-DecaXP. The Voyager is a MALDI-TOF (matrix assisted laser desorption ionization with a time-of-flight analyzer) workstation with delayed extraction technology. It has a mass range of up to 500,000 Da for singly charged ions. The QSTAR is an electrospray ionization mass spectrometer with a TOF analyzer, and can detect masses up to 12,000 Da for singly charged ions. The LCQ also is an electrospray ionization mass spectrometer with an ion trap analyzer with a mass range of up to 4000 Da for singly charged ions. All instruments have high sensitivity (low fmol for peptides) and high resolution. The two high pressure liquid chromatography (HPLC) systems (Surveyor HPLC system from Thermo Finnigan and Ultimate/Switchos/Famos HPLC system from LC Packings/Dionex) are interfaced with the electrospray ionization mass spectrometers for microflow or nanoflow applications (high sensitivity) and on-line fragmentation, sequencing and identification of peptides. The facility also has a ProteomeX Workstation (Thermo Finnigan), a fully integrated multi-dimensional chromatography HPLC system interfaced with a LCQ ion trap mass spectrometer, and two dimensional (2D) gel electrophoresis equipment, gel spot handling robotics, gel imaging scanners (Amersham Biosciences). Our facility is equipped to analyze by mass spectrometry proteins, peptides, oligonucleotides, lipids, carbohydrates and small molecules from samples in solution or separated by chromatography or electrophoresis. In addition to conventional electrophoresis and chromatography fractionation, the facility will offer differential analysis of protein samples by Differential Imaging Gel Electrophoresis (DIGETM, Amersham Biosciences) analysis and Isotope-Coded Affinity Tag (ICATTM, Applied Biosystems) analysis.
Contact:
Robert N. Cole, Director
Chad Bradford, Lab Manager
410-614-6968 (Office)
410-614-6968 (Lab)
rcole@jhmi.edu, cbradford@jhmi.edu
http://www.hopkinsmedicine.org/msf
Protein and DNA Segmenting
- The DNA Analysis Facility of the Genetic Resources Core Facility
The source for all of your DNA sequencing and synthesis needs.
Contact:
Sequencing: 410-955-2836
Synthesis: 410-614-3649
Fax: 410-614-3148
http://www.daf.jhmi.edu/
- The Fragment Analysis Facility of the Genetic Resources Core Facility
See Genotyping
- The Synthesis and Sequencing Facility
See Proteometrics
- AB Mass Spectrometry/Proteomic Facility
See Proteometrics
Nuclear Magnetic Resonance
- Biomolecular NMR Center
Advanced biomolecular research: structural studies of proteins, nucleic acids, carbohydrates. Instruments include: Varian Inova 800 MHz NMR spectrometer, Varian Inova 500 MHz NMR spectrometer, Bruker Avance 600 MHz NMR spectrometer.
Varian Unity 500 MHz FT-NMR spectrometer, Varian Unity 400 MHz FT-NMR spectrometer, Bruker AMX-300 300 MHz FT-NMR spectrometer, Varian Mercury 200 200 MHz FT-NMR spectrometer (in the undergraduate instructional laboratory)
Contact:
Ananya Majumdar, Director
(410) 516-8670
ananya@jhu.edu